The Spanish royalty were visiting the Coslada Transport Center in Madrid when they payed their respects to those who have died from the coronavirus.
Spain has been one of the hardest hit nations in Europe with 27,134 coronavirus-related deaths reported and the country went into a 10-day mourning period on Wednesday last week.
The country has announced zero new deaths from the disease for the second day running as lockdown restrictions are set to be eased next week.
Lockdown restrictions in the hard-hit cities of Madrid and Barcelona will be eased further, Spanish health minister Salvador Illa told a news conference.
Customers will be allowed to eat and drink inside bars and restaurants rather than just on outdoor terraces and children will be able to play outside at any time of day from Monday.
Queen Letizia wore an all-black ensemble while mourning the country's victims and her husband wore a black tie.
Both of the Royals donned personal-protective equipment (PPE) face masks while walking around the Transport Center, as did staff.
