The gunman, who went on a deadly rampage killed two people and injured three before turning the gun on himself.
Dangelo Dorsey had followed through a series of carjackings and shootings before fleeing from the police on September 13.
The horrific rampage ended with a high-speed car chase in Tennessee.
He ultimately shot himself in front of the pregnant wife of a man he had killed earlier after holding them hostage when he carjacked them as they went to church.
David Rausch, director of The Tennessee Bureau of Investigation, said in a press release that the killing spree started at 9.30am when the 29-year-old opened fire inside a moving vehicle along Interstate 24 near Beechgrove.
Dorsey, from St. Louis, Missouri, got out of the vehicle as traffic came to a halt shooting a woman in her hand as he attempted to carjack her vehicle and he fatally shot a nearby truck driver in the face.
Aileen Stevens was teaching Sunday School that morning at her church in Rutherford County and was on her way there with husband Jordan in their Ford Mustang when they were rear-ended by Dorsey.
Mr Rausch said the couple were forced to drive back to their home at gunpoint by Dorsey who is claimed to have stolen two of their guns before he made them swap cars and go back out with him.
Police say at some point Mr Stevens was shot dead in front of his wife by Dorsey who dumped the body before driving off with the terrified mum-to-be.
Officers managed to pinpoint the SUV they were in and a 100mph police chase began before the car crashed and flipped.
Mr Rausch said Dorsey then shot himself dead in front of Mrs Stevens.
He added: "The female hostage was safely recovered."
